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anti collagen type iii antibody  (Boster Bio)


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    Structured Review

    Boster Bio anti collagen type iii antibody
    Anti Collagen Type Iii Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+collagen/Anti-Collagen+III+Rabbit+Monoclonal+Antibody/pm41998635-158-5-13
    Average 93 stars, based on 48 article reviews
    anti collagen type iii antibody - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Staining:

    Article Title: Tissue-Engineered Corneal Endothelial Sheets Using Ultrathin Acellular Porcine Corneal Stroma Substrates for Endothelial Keratoplasty.
    Article Snippet: .. For IF staining analysis, the sections and grafts were blocked in goat serum for 30 min and incubated with rabbit anti-collagen I (1:200, Boster, CA), mouse anticollagen IV (1:20, Novus Biologicals, Colorado), mouse polyclonal anti-Na+/K+-ATPase (1:100, Millipore, Billerica, MA), mouse monoclonal anti-ZO-1 (1:100, Invitrogen), and mouse anti-Laminin (1:100, Millipore) overnight at 4 °C, and then incubated in the corresponding secondary antibodies (1:100) for 2 h at room temperature. ..

    Incubation:

    Article Title: Tissue-Engineered Corneal Endothelial Sheets Using Ultrathin Acellular Porcine Corneal Stroma Substrates for Endothelial Keratoplasty.
    Article Snippet: .. For IF staining analysis, the sections and grafts were blocked in goat serum for 30 min and incubated with rabbit anti-collagen I (1:200, Boster, CA), mouse anticollagen IV (1:20, Novus Biologicals, Colorado), mouse polyclonal anti-Na+/K+-ATPase (1:100, Millipore, Billerica, MA), mouse monoclonal anti-ZO-1 (1:100, Invitrogen), and mouse anti-Laminin (1:100, Millipore) overnight at 4 °C, and then incubated in the corresponding secondary antibodies (1:100) for 2 h at room temperature. ..

    Article Title: β-Actin: Not a Suitable Internal Control of Hepatic Fibrosis Caused by Schistosoma japonicum
    Article Snippet: Then BCA protein assay kit (Beyotime Biotechnology, Shanghai, China) was used to determine the concentration of total protein. .. Equal amounts (60 μg) of protein were loaded and separated on a 10% polyacrylamide gel with 200 V for 2 h and then transferred to a 0.22 μm PVDF membrane (Millipore, MA, United States) with 300 mA for 3 h. After blocking in PBS containing 5% milk and 0.1% Tween-20 for 2 h at room temperature, the membranes were incubated with primary antibodies mouse anti-β-actin (58169, Cell Signaling Technology, Boston, MA, United States, used at 1:2000 dilution), mouse anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (G8795, Sigma-Aldrich, St. Louis, MO, United States, used at 1:2000 dilution), and mouse anti-β-Tubulin (T0198, Sigma-Aldrich, St. Louis, MO, United States, used at 1:2000 dilution), rabbit anti-α-SMA (19245, Cell Signaling Technology, Boston, MA, United States, used at 1:1000 dilution), rabbit anti-Collagen I (COL1A1) (BA0325, Boster, Wuhan, China, used at 1:1000 dilution), rabbit anti-Collagen III (COL3A1) (BM1625, Boster, Wuhan, China, used at 1:1000 dilution) at 4°C overnight. ..

    Article Title: β-Actin: Not a Suitable Internal Control of Hepatic Fibrosis Caused by Schistosoma japonicum .
    Article Snippet: Then BCA protein assay kit (Beyotime Biotechnology, Shanghai, China) was used to determine the concentration of total protein. .. Equal amounts (60 μg) of protein were loaded and separated on a 10% polyacrylamide gel with 200 V for 2 h and then transferred to a 0.22 μm PVDF membrane (Millipore, MA, United States) with 300 mA for 3 h. After blocking in PBS containing 5% milk and 0.1% Tween-20 for 2 h at room temperature, the membranes were incubated with primary antibodies mouse anti-β-actin (58169, Cell Signaling Technology, Boston, MA, United States, used at 1:2000 dilution), mouse anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (G8795, Sigma-Aldrich, St. Louis, MO, United States, used at 1:2000 dilution), and mouse antiβ-Tubulin (T0198, Sigma-Aldrich, St. Louis, MO, United States, used at 1:2000 dilution), rabbit anti-α-SMA (19245, Cell Signaling Technology, Boston, MA, United States, used at 1:1000 dilution), rabbit anti-Collagen I (COL1A1) (BA0325, Boster, Wuhan, China, used at 1:1000 dilution), rabbit antiCollagen III (COL3A1) (BM1625, Boster, Wuhan, China, used at 1:1000 dilution) at 4◦C overnight. ..

    Membrane:

    Article Title: β-Actin: Not a Suitable Internal Control of Hepatic Fibrosis Caused by Schistosoma japonicum
    Article Snippet: Then BCA protein assay kit (Beyotime Biotechnology, Shanghai, China) was used to determine the concentration of total protein. .. Equal amounts (60 μg) of protein were loaded and separated on a 10% polyacrylamide gel with 200 V for 2 h and then transferred to a 0.22 μm PVDF membrane (Millipore, MA, United States) with 300 mA for 3 h. After blocking in PBS containing 5% milk and 0.1% Tween-20 for 2 h at room temperature, the membranes were incubated with primary antibodies mouse anti-β-actin (58169, Cell Signaling Technology, Boston, MA, United States, used at 1:2000 dilution), mouse anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (G8795, Sigma-Aldrich, St. Louis, MO, United States, used at 1:2000 dilution), and mouse anti-β-Tubulin (T0198, Sigma-Aldrich, St. Louis, MO, United States, used at 1:2000 dilution), rabbit anti-α-SMA (19245, Cell Signaling Technology, Boston, MA, United States, used at 1:1000 dilution), rabbit anti-Collagen I (COL1A1) (BA0325, Boster, Wuhan, China, used at 1:1000 dilution), rabbit anti-Collagen III (COL3A1) (BM1625, Boster, Wuhan, China, used at 1:1000 dilution) at 4°C overnight. ..

    Article Title: β-Actin: Not a Suitable Internal Control of Hepatic Fibrosis Caused by Schistosoma japonicum .
    Article Snippet: Then BCA protein assay kit (Beyotime Biotechnology, Shanghai, China) was used to determine the concentration of total protein. .. Equal amounts (60 μg) of protein were loaded and separated on a 10% polyacrylamide gel with 200 V for 2 h and then transferred to a 0.22 μm PVDF membrane (Millipore, MA, United States) with 300 mA for 3 h. After blocking in PBS containing 5% milk and 0.1% Tween-20 for 2 h at room temperature, the membranes were incubated with primary antibodies mouse anti-β-actin (58169, Cell Signaling Technology, Boston, MA, United States, used at 1:2000 dilution), mouse anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (G8795, Sigma-Aldrich, St. Louis, MO, United States, used at 1:2000 dilution), and mouse antiβ-Tubulin (T0198, Sigma-Aldrich, St. Louis, MO, United States, used at 1:2000 dilution), rabbit anti-α-SMA (19245, Cell Signaling Technology, Boston, MA, United States, used at 1:1000 dilution), rabbit anti-Collagen I (COL1A1) (BA0325, Boster, Wuhan, China, used at 1:1000 dilution), rabbit antiCollagen III (COL3A1) (BM1625, Boster, Wuhan, China, used at 1:1000 dilution) at 4◦C overnight. ..

    Blocking Assay:

    Article Title: β-Actin: Not a Suitable Internal Control of Hepatic Fibrosis Caused by Schistosoma japonicum
    Article Snippet: Then BCA protein assay kit (Beyotime Biotechnology, Shanghai, China) was used to determine the concentration of total protein. .. Equal amounts (60 μg) of protein were loaded and separated on a 10% polyacrylamide gel with 200 V for 2 h and then transferred to a 0.22 μm PVDF membrane (Millipore, MA, United States) with 300 mA for 3 h. After blocking in PBS containing 5% milk and 0.1% Tween-20 for 2 h at room temperature, the membranes were incubated with primary antibodies mouse anti-β-actin (58169, Cell Signaling Technology, Boston, MA, United States, used at 1:2000 dilution), mouse anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (G8795, Sigma-Aldrich, St. Louis, MO, United States, used at 1:2000 dilution), and mouse anti-β-Tubulin (T0198, Sigma-Aldrich, St. Louis, MO, United States, used at 1:2000 dilution), rabbit anti-α-SMA (19245, Cell Signaling Technology, Boston, MA, United States, used at 1:1000 dilution), rabbit anti-Collagen I (COL1A1) (BA0325, Boster, Wuhan, China, used at 1:1000 dilution), rabbit anti-Collagen III (COL3A1) (BM1625, Boster, Wuhan, China, used at 1:1000 dilution) at 4°C overnight. ..

    Article Title: β-Actin: Not a Suitable Internal Control of Hepatic Fibrosis Caused by Schistosoma japonicum .
    Article Snippet: Then BCA protein assay kit (Beyotime Biotechnology, Shanghai, China) was used to determine the concentration of total protein. .. Equal amounts (60 μg) of protein were loaded and separated on a 10% polyacrylamide gel with 200 V for 2 h and then transferred to a 0.22 μm PVDF membrane (Millipore, MA, United States) with 300 mA for 3 h. After blocking in PBS containing 5% milk and 0.1% Tween-20 for 2 h at room temperature, the membranes were incubated with primary antibodies mouse anti-β-actin (58169, Cell Signaling Technology, Boston, MA, United States, used at 1:2000 dilution), mouse anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (G8795, Sigma-Aldrich, St. Louis, MO, United States, used at 1:2000 dilution), and mouse antiβ-Tubulin (T0198, Sigma-Aldrich, St. Louis, MO, United States, used at 1:2000 dilution), rabbit anti-α-SMA (19245, Cell Signaling Technology, Boston, MA, United States, used at 1:1000 dilution), rabbit anti-Collagen I (COL1A1) (BA0325, Boster, Wuhan, China, used at 1:1000 dilution), rabbit antiCollagen III (COL3A1) (BM1625, Boster, Wuhan, China, used at 1:1000 dilution) at 4◦C overnight. ..

    Western Blot:

    Article Title: Bimodal fibrosis in a novel mouse model of bleomycin-induced usual interstitial pneumonia
    Article Snippet: .. For Western blot, the following primary antibodies were used: rabbit anti-collagen (Boster biological technology), rabbit anti-αSMA, rabbit anti-vimentin, rabbit anti-PDGFRα, rabbit anti-PDGFRβ and rabbit anti-E-cadherin (Cell Signaling Technology), goat anti-SP-D, mouse anti-TGF-β and goat anti-Serpin F1/PEDF (R&D Systems), rabbit anti-Uteroglobin, Rabbit anti-CD31 and rabbit anti-VEGFA (Abcam), rabbit anti-SP-A, mouse anti-PDGFA and mouse anti-Tubulin-α (Santa Cruz), rabbit anti-SP-C (Hycult Biotech) and rabbit anti-PDGFB (Bioss antibodies). .. ECLTM anti-rabbit or anti-rat IgG, horseradish peroxidase linked antibodies were used as a secondary antibody (GE Healthcare).



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    OriGene primary antibodies for col1a1
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    Image Search Results


    TGF-β secreted from the hiPSC-derived CM patch induced Col1a1 expression but not Col3a1 (A) Schematic representation of the co-culture system. hiPSC-derived CMs were seeded on the upper chamber, whereas the cardiac fibroblasts isolated from mice heart were cultured at the lower chamber in the presence and absence of SB431542, a TGF-β receptor inhibitor. (B) RT-qPCR analysis of Col1a1 , Col3a1 , and Pai1 in the cultured cardiac fibroblasts. Two-way ANOVA (co-culture × inhibitor) with interaction; Tukey-adjusted post hoc tests on estimated marginal means. Data represent mean ± SEM. N = 3–6, one-way ANOVA followed by Tukey’s HSD test, p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

    Journal: iScience

    Article Title: Human iPSC cardiomyocyte patch transplantation modifies extracellular matrix and fibroblast behavior after myocardial infarction

    doi: 10.1016/j.isci.2026.115341

    Figure Lengend Snippet: TGF-β secreted from the hiPSC-derived CM patch induced Col1a1 expression but not Col3a1 (A) Schematic representation of the co-culture system. hiPSC-derived CMs were seeded on the upper chamber, whereas the cardiac fibroblasts isolated from mice heart were cultured at the lower chamber in the presence and absence of SB431542, a TGF-β receptor inhibitor. (B) RT-qPCR analysis of Col1a1 , Col3a1 , and Pai1 in the cultured cardiac fibroblasts. Two-way ANOVA (co-culture × inhibitor) with interaction; Tukey-adjusted post hoc tests on estimated marginal means. Data represent mean ± SEM. N = 3–6, one-way ANOVA followed by Tukey’s HSD test, p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

    Article Snippet: Then proteins were analyzed using primary antibodies for Col1a1 (R1038, OriGene, Rockville, MD), Col3a1(ab6310, Abcam, Cambridge, UK) and Gapdh (AM4300, Thermo Fisher Scienctific, Waltham, MA), respectively on Amersham Imager 600 (GE Healthcare, Chicago, IL).

    Techniques: Derivative Assay, Expressing, Co-Culture Assay, Isolation, Cell Culture, Quantitative RT-PCR