anti collagen type iii antibody (Boster Bio)
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Anti Collagen Type Iii Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+collagen/Anti-Collagen+III+Rabbit+Monoclonal+Antibody/pm41998635-158-5-13
Average 93 stars, based on 48 article reviews
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Staining:Article Title: Tissue-Engineered Corneal Endothelial Sheets Using Ultrathin Acellular Porcine Corneal Stroma Substrates for Endothelial Keratoplasty. Article Snippet: .. For IF staining analysis, the sections and grafts were blocked in goat serum for 30 min and incubated with Incubation:Article Title: Tissue-Engineered Corneal Endothelial Sheets Using Ultrathin Acellular Porcine Corneal Stroma Substrates for Endothelial Keratoplasty. Article Snippet: .. For IF staining analysis, the sections and grafts were blocked in goat serum for 30 min and incubated with Article Title: β-Actin: Not a Suitable Internal Control of Hepatic Fibrosis Caused by Schistosoma japonicum Article Snippet: Then BCA protein assay kit (Beyotime Biotechnology, Shanghai, China) was used to determine the concentration of total protein. .. Equal amounts (60 μg) of protein were loaded and separated on a 10% polyacrylamide gel with 200 V for 2 h and then transferred to a 0.22 μm PVDF membrane (Millipore, MA, United States) with 300 mA for 3 h. After blocking in PBS containing 5% milk and 0.1% Tween-20 for 2 h at room temperature, the membranes were incubated with primary antibodies mouse anti-β-actin (58169, Cell Signaling Technology, Boston, MA, United States, used at 1:2000 dilution), mouse anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (G8795, Sigma-Aldrich, St. Louis, MO, United States, used at 1:2000 dilution), and mouse anti-β-Tubulin (T0198, Sigma-Aldrich, St. Louis, MO, United States, used at 1:2000 dilution), rabbit anti-α-SMA (19245, Cell Signaling Technology, Boston, MA, United States, used at 1:1000 dilution), Article Title: β-Actin: Not a Suitable Internal Control of Hepatic Fibrosis Caused by Schistosoma japonicum . Article Snippet: Then BCA protein assay kit (Beyotime Biotechnology, Shanghai, China) was used to determine the concentration of total protein. .. Equal amounts (60 μg) of protein were loaded and separated on a 10% polyacrylamide gel with 200 V for 2 h and then transferred to a 0.22 μm PVDF membrane (Millipore, MA, United States) with 300 mA for 3 h. After blocking in PBS containing 5% milk and 0.1% Tween-20 for 2 h at room temperature, the membranes were incubated with primary antibodies mouse anti-β-actin (58169, Cell Signaling Technology, Boston, MA, United States, used at 1:2000 dilution), mouse anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (G8795, Sigma-Aldrich, St. Louis, MO, United States, used at 1:2000 dilution), and mouse antiβ-Tubulin (T0198, Sigma-Aldrich, St. Louis, MO, United States, used at 1:2000 dilution), rabbit anti-α-SMA (19245, Cell Signaling Technology, Boston, MA, United States, used at 1:1000 dilution), Membrane:Article Title: β-Actin: Not a Suitable Internal Control of Hepatic Fibrosis Caused by Schistosoma japonicum Article Snippet: Then BCA protein assay kit (Beyotime Biotechnology, Shanghai, China) was used to determine the concentration of total protein. .. Equal amounts (60 μg) of protein were loaded and separated on a 10% polyacrylamide gel with 200 V for 2 h and then transferred to a 0.22 μm PVDF membrane (Millipore, MA, United States) with 300 mA for 3 h. After blocking in PBS containing 5% milk and 0.1% Tween-20 for 2 h at room temperature, the membranes were incubated with primary antibodies mouse anti-β-actin (58169, Cell Signaling Technology, Boston, MA, United States, used at 1:2000 dilution), mouse anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (G8795, Sigma-Aldrich, St. Louis, MO, United States, used at 1:2000 dilution), and mouse anti-β-Tubulin (T0198, Sigma-Aldrich, St. Louis, MO, United States, used at 1:2000 dilution), rabbit anti-α-SMA (19245, Cell Signaling Technology, Boston, MA, United States, used at 1:1000 dilution), Article Title: β-Actin: Not a Suitable Internal Control of Hepatic Fibrosis Caused by Schistosoma japonicum . Article Snippet: Then BCA protein assay kit (Beyotime Biotechnology, Shanghai, China) was used to determine the concentration of total protein. .. Equal amounts (60 μg) of protein were loaded and separated on a 10% polyacrylamide gel with 200 V for 2 h and then transferred to a 0.22 μm PVDF membrane (Millipore, MA, United States) with 300 mA for 3 h. After blocking in PBS containing 5% milk and 0.1% Tween-20 for 2 h at room temperature, the membranes were incubated with primary antibodies mouse anti-β-actin (58169, Cell Signaling Technology, Boston, MA, United States, used at 1:2000 dilution), mouse anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (G8795, Sigma-Aldrich, St. Louis, MO, United States, used at 1:2000 dilution), and mouse antiβ-Tubulin (T0198, Sigma-Aldrich, St. Louis, MO, United States, used at 1:2000 dilution), rabbit anti-α-SMA (19245, Cell Signaling Technology, Boston, MA, United States, used at 1:1000 dilution), Blocking Assay:Article Title: β-Actin: Not a Suitable Internal Control of Hepatic Fibrosis Caused by Schistosoma japonicum Article Snippet: Then BCA protein assay kit (Beyotime Biotechnology, Shanghai, China) was used to determine the concentration of total protein. .. Equal amounts (60 μg) of protein were loaded and separated on a 10% polyacrylamide gel with 200 V for 2 h and then transferred to a 0.22 μm PVDF membrane (Millipore, MA, United States) with 300 mA for 3 h. After blocking in PBS containing 5% milk and 0.1% Tween-20 for 2 h at room temperature, the membranes were incubated with primary antibodies mouse anti-β-actin (58169, Cell Signaling Technology, Boston, MA, United States, used at 1:2000 dilution), mouse anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (G8795, Sigma-Aldrich, St. Louis, MO, United States, used at 1:2000 dilution), and mouse anti-β-Tubulin (T0198, Sigma-Aldrich, St. Louis, MO, United States, used at 1:2000 dilution), rabbit anti-α-SMA (19245, Cell Signaling Technology, Boston, MA, United States, used at 1:1000 dilution), Article Title: β-Actin: Not a Suitable Internal Control of Hepatic Fibrosis Caused by Schistosoma japonicum . Article Snippet: Then BCA protein assay kit (Beyotime Biotechnology, Shanghai, China) was used to determine the concentration of total protein. .. Equal amounts (60 μg) of protein were loaded and separated on a 10% polyacrylamide gel with 200 V for 2 h and then transferred to a 0.22 μm PVDF membrane (Millipore, MA, United States) with 300 mA for 3 h. After blocking in PBS containing 5% milk and 0.1% Tween-20 for 2 h at room temperature, the membranes were incubated with primary antibodies mouse anti-β-actin (58169, Cell Signaling Technology, Boston, MA, United States, used at 1:2000 dilution), mouse anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (G8795, Sigma-Aldrich, St. Louis, MO, United States, used at 1:2000 dilution), and mouse antiβ-Tubulin (T0198, Sigma-Aldrich, St. Louis, MO, United States, used at 1:2000 dilution), rabbit anti-α-SMA (19245, Cell Signaling Technology, Boston, MA, United States, used at 1:1000 dilution), Western Blot:Article Title: Bimodal fibrosis in a novel mouse model of bleomycin-induced usual interstitial pneumonia Article Snippet: .. For Western blot, the following primary antibodies were used: |
